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Cyanobacterial assimilatory nitrate reductase gene diversity in coastal and oligotrophic marine environments
Author(s) -
Jenkins Bethany D.,
Zehr Jonathan P.,
Gibson Angela,
Campbell Lisa
Publication year - 2006
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/j.1462-2920.2006.01084.x
Subject(s) - biology , nitrate reductase , synechococcus , cyanobacteria , phylogenetic tree , bay , nitrogen assimilation , ribosomal rna , ecology , phylogenetic diversity , gene , nitrate , botany , genetics , bacteria , oceanography , geology
Summary Cyanobacteria are important primary producers in many marine ecosystems and their abundances and growth rates depend on their ability to assimilate various nitrogen sources. To examine the diversity of nitrate‐utilizing marine cyanobacteria, we developed PCR primers specific for cyanobacterial assimilatory nitrate reductase ( narB ) genes. We obtained amplification products from diverse strains of cultivated cyanobacteria and from several marine environments. Phylogenetic trees constructed with the narB gene are congruent with those based on ribosomal RNA genes and RNA polymerase genes. Analysis of sequence library data from coastal and oligotrophic marine environments shows distinct groups of Synechococcus sp. in each environment; some of which are represented by sequences from cultivated organisms and others that are unrelated to known sequences and likely represent novel phylogenetic groups. We observed spatial differences in the distribution of sequences between two sites in Monterey Bay and differences in the vertical distribution of sequence types at the Hawai’i Ocean Time‐series Station ALOHA, suggesting that nitrogen assimilation in Synechococcus living in different ecological niches can be followed with the nitrate reductase gene.