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Recovery of temperate Desulfovibrio vulgaris bacteriophage using a novel host strain
Author(s) -
Walker Christopher B.,
Stolyar Sergey S.,
Pinel Nic,
Yen HueiChe B.,
He Zhili,
Zhou Jizhong,
Wall Judy D.,
Stahl David A.
Publication year - 2006
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/j.1462-2920.2006.01075.x
Subject(s) - desulfovibrio vulgaris , biology , strain (injury) , bacteriophage , genome , pulsed field gel electrophoresis , microbiology and biotechnology , temperateness , bacteria , gene , genetics , escherichia coli , genotype , anatomy
Summary A novel sulfate‐reducing bacterium (strain DePue) closely related to Desulfovibrio vulgaris ssp. vulgaris strain Hildenborough was isolated from the sediment of a heavy‐metal impacted lake using established techniques. Although few physiological differences between strains DePue and Hildenborough were observed, pulse‐field gel electrophoresis (PFGE) revealed a significant genome reduction in strain DePue. Comparative whole‐genome microarray and polymerase chain reaction analyses demonstrated that the absence of genes annotated in the Hildenborough genome as phage or phage‐related contributed to the significant genome reduction in strain DePue. Two morphotypically distinct temperate bacteriophage from strain Hildenborough were recovered using strain DePue as a host for plaque isolation.