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Growth phase‐dependent expression of the Pseudomonas putida KT2440 transcriptional machinery analysed with a genome‐wide DNA microarray
Author(s) -
Yuste Luis,
Hervás Ana B.,
Canosa Inés,
Tobes Raquel,
Jiménez José Ignacio,
Nogales Juan,
PérezPérez Manuel M.,
Santero Eduardo,
Díaz Eduardo,
Ramos JuanLuis,
De Lorenzo Víctor,
Rojo Fernando
Publication year - 2006
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/j.1462-2920.2005.00890.x
Subject(s) - sigma factor , biology , pseudomonas putida , rna polymerase , gene , genetics , transcriptional regulation , gene expression , microbiology and biotechnology , rna
Summary Bacterial transcriptional networks are built on a hierarchy of regulators, on top of which lie the components of the RNA polymerase (in particular the sigma factors) and the global control elements, which play a pivotal role. We have designed a genome‐wide oligonucleotide‐based DNA microarray for Pseudomonas putida KT2440. In combination with real‐time reverse transcription polymerase chain reaction (RT‐PCR), we have used it to analyse the expression pattern of the genes encoding the RNA polymerase subunits (the core enzyme and the 24 sigma factors), and various proteins involved in global regulation (Crc, Lrp, Fur, Anr, Fis, CsrA, IHF, HupA, HupB, HupN, BipA and several MvaT‐like proteins), during the shift from exponential growth in rich medium into starvation and stress brought about by the entry into stationary phase. Expression of the genes encoding the RNA polymerase core and the vegetative sigma factor decreased in stationary phase, while that of σ S increased. Data obtained for σ N , σ H , FliA and for the 19 extracytoplasmic function (ECF)‐like sigma factors suggested that their mRNA levels change little upon entry into stationary phase. Expression of Crc, BipA, Fis, HupB, HupN and the MvaT‐like protein PP3693 decreased in stationary phase, while that of HupA and the MvaT‐like protein PP3765 increased significantly. Expression of IHF was indicative of post‐transcriptional control. These results provide the first global study of the expression of the transcriptional machinery through the exponential stationary‐phase shift in P. putida .