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Diversity of N‐acyl homoserine lactone‐producing and ‐degrading bacteria in soil and tobacco rhizosphere
Author(s) -
D’AngeloPicard Cathy,
Faure Denis,
Penot Isabelle,
Dessaux Yves
Publication year - 2005
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/j.1462-2920.2005.00886.x
Subject(s) - biology , sphingomonas , rhizosphere , proteobacteria , microbiology and biotechnology , bacteria , sinorhizobium , homoserine , 16s ribosomal rna , restriction fragment length polymorphism , pseudomonas , botany , genotype , quorum sensing , genetics , rhizobiaceae , symbiosis , gene , biofilm
Summary In Gram‐negative bacteria, quorum‐sensing (QS) communication is mostly mediated by N‐acyl homoserine lactones (N‐AHSL). The diversity of bacterial populations that produce or inactivate the N‐AHSL signal in soil and tobacco rhizosphere was investigated by restriction fragment length polymorphism (RFLP) analysis of amplified 16S DNA and DNA sequencing. Such analysis indicated the occurrence of N‐AHSL‐producing strains among the α‐, β‐ and γ‐proteobacteria, including genera known to produce N‐AHSL ( Rhizobium , Sinorhizobium and Pseudomonas ) and novel genera with no previously identified N‐AHSL‐producing isolates ( Variovorax , Sphingomonas and Massilia ). The diversity of N‐AHSL signals was also investigated in relation to the genetic diversity of the isolates. However, N‐AHSL‐degrading strains isolated from soil samples belonged to the Bacillus genus, while strains isolated from tobacco rhizospheres belonged to both the Bacillus genus and to the α subgroup of proteobacteria, suggesting that diversity of N‐AHSL‐degrading strains may be modulated by the presence of the tobacco plant. Among these rhizospheric isolates, novel N‐AHSL‐degrading genera have been identified ( Sphingomonas and Bosea ). As the first simultaneous analysis of both N‐AHSL‐degrading and ‐producing bacterial communities in a complex environment, this study revealed the coexistence of bacterial isolates, belonging to the same genus or species that may produce or degrade N‐AHSL.