Premium
Isolation and characterization of Pseudoalteromonas strains with divergent polycyclic aromatic hydrocarbon catabolic properties
Author(s) -
Hedlund Brian P.,
Staley James T.
Publication year - 2006
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/j.1462-2920.2005.00871.x
Subject(s) - phenanthrene , naphthalene , pseudoalteromonas , biology , 16s ribosomal rna , polycyclic aromatic hydrocarbon , bacteria , microbiology and biotechnology , gene , hydrocarbon , isolation (microbiology) , aromatic hydrocarbon , biochemistry , genetics , chemistry , organic chemistry , astrobiology , catalysis
Summary Fifteen strains of polycyclic aromatic hydrocarbon (PAH)‐catabolizing bacteria, identified as Pseudoalteromonas spp. were isolated from Eagle Harbor, Puget Sound, USA, using a most probable number procedure in which naphthalene or phenanthrene was the sole carbon and energy source. Despite having identical 16S rDNA sequences, some catabolized many PAHs, whereas others oxidized only naphthalenes. A putative naphthalene 1,2‐dioxygenase gene fragment was polymerase chain reaction‐amplified from the naphthalene‐degrading strains and shown to be almost identical to a gene present in Neptunomonas naphthovorans , suggesting horizontal transfer.