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The GluR2 subunit inhibits proliferation by inactivating Src‐MAPK signalling and induces apoptosis by means of caspase 3/6‐dependent activation in glioma cells
Author(s) -
Beretta Francesca,
Bassani Silvia,
Binda Elena,
Verpelli Chiara,
Bello Lorenzo,
Galli Rossella,
Passafaro Maria
Publication year - 2009
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.2009.06804.x
Subject(s) - glioma , mapk/erk pathway , proto oncogene tyrosine protein kinase src , kinase , apoptosis , cancer research , microbiology and biotechnology , protein subunit , chemistry , phosphorylation , caspase 3 , downregulation and upregulation , biology , programmed cell death , biochemistry , gene
Glioblastoma multiforme (GBM) is the most invasive and undifferentiated type of brain tumour, and so surgical interventions are ineffective. We found that GluR2 is absent in fast‐growing GBM‐derived tumour stem cells and high‐grade glioma specimens, but is expressed in slow‐growing stem cells and low‐grade glioma specimens. More remarkably, GluR2 overexpression in U‐87MG cells inhibits proliferation by inactivating extracellular signal‐regulated kinase (ERK)1/2‐Src phosphorylation and induces apoptosis. Mechanistically, we observed that the scaffold protein GRIP is essential for the effect of GluR2 on ERK‐Src inactivation. These findings indicate that the absence of the GluR2 subunit favours malignancy.