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Activation of transient receptor potential ankyrin 1 by hydrogen peroxide
Author(s) -
Sawada Yosuke,
Hosokawa Hiroshi,
Matsumura Kiyoshi,
Kobayashi Shigeo
Publication year - 2008
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.2008.06093.x
Subject(s) - transient receptor potential channel , chemistry , ankyrin , reactive oxygen species , hydrogen peroxide , trpv1 , receptor , cysteine , microbiology and biotechnology , isothiocyanate , dorsal root ganglion , oxidizing agent , biochemistry , biophysics , sensory system , neuroscience , biology , enzyme , gene , organic chemistry
Hydrogen peroxide (H 2 O 2 ), which is contained in industrial products, is also generated within cells. H 2 O 2 causes pain but it has not been elucidated how it activates sensory neurons in the pain pathway. Here we show that transient receptor potential ankyrin 1 (TRPA1), expressed by sensory neurons in the pain pathway, is a receptor for H 2 O 2 . H 2 O 2 activated mouse TRPA1 to induce Ca 2+ influx and elicit non‐selective cation currents. These effects of H 2 O 2 were mimicked by both reactive oxygen species and reactive nitrogen species. Cysteine‐reducing agents suppressed H 2 O 2 ‐induced TRPA1 activation, whereas cysteine‐oxidizing agents activated TRPA1. H 2 O 2 caused Ca 2+ influx in a subset of dorsal root ganglia neurons, which responded to allyl isothiocyanate, a TRPA1 ligand. These results suggest that TRPA1 might be involved in the sensation of pain caused by H 2 O 2 .