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The chemical component of the mixed GF‐TTMn synapse in Drosophila melanogaster uses acetylcholine as its neurotransmitter
Author(s) -
Allen Marcus J.,
Murphey R. K.
Publication year - 2007
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.2007.05686.x
Subject(s) - synapse , cholinergic , neurotransmitter , neuromuscular junction , neurotransmission , acetylcholine , choline acetyltransferase , biology , drosophila melanogaster , neuroscience , microbiology and biotechnology , mutant , excitatory synapse , biochemistry , receptor , gene , central nervous system , endocrinology
The largest central synapse in adult Drosophila is a mixed electro‐chemical synapse whose gap junctions require the product of the shaking‐B (shak‐B) gene. Shak‐B 2 mutant flies lack gap junctions at this synapse, which is between the giant fibre (GF) and the tergotrochanteral motor neuron (TTMn), but it still exhibits a long latency response upon GF stimulation. We have targeted the expression of the light chain of tetanus toxin to the GF, to block chemical transmission, in shak‐B 2 flies. The long latency response in the tergotrochanteral muscle (TTM) was abolished indicating that the chemical component of the synapse mediates this response. Attenuation of GAL4‐mediated labelling by a cha‐GAL80 transgene, reveals the GF to be cholinergic. We have used a temperature‐sensitive allele of the choline acetyltransferase gene ( cha ts2 ) to block cholinergic synapses in adult flies and this also abolished the long latency response in shak‐B 2 flies. Taken together the data provide evidence that both components of this mixed synapse are functional and that the chemical neurotransmitter between the GF and the TTMn is acetylcholine. Our findings show that the two components of this synapse can be separated to allow further studies into the mechanisms by which mixed synapses are built and function.