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An antibody to synaptotagmin I facilitates synaptic transmission
Author(s) -
Hua ShaoYing,
Teylan Merilee A.,
Cimenser Aylin
Publication year - 2007
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.2007.05602.x
Subject(s) - synaptotagmin 1 , synaptotagmin i , neurotransmitter , neurotransmission , synaptic vesicle , stx1a , neuroscience , synaptic fatigue , neuromuscular junction , chemistry , biology , microbiology and biotechnology , biophysics , inhibitory postsynaptic potential , central nervous system , excitatory postsynaptic potential , biochemistry , vesicle , receptor , membrane
Proper functioning of the nervous system requires precise control of neurotransmitter release. Synaptotagmin, a synaptic vesicle protein, is crucial for the temporal control of neurotransmitter release. The mechanism of synaptotagmin function is still under debate. To investigate the mechanism by which synaptotagmin controls neurotransmitter release, we injected an antibody of rat synaptotagmin I into a crayfish motor axon. We found that the antibody enhanced synaptic transmission at crayfish neuromuscular junctions by increasing the amplitude of the evoked synaptic response. This effect was antibody‐dose dependent. The antibody also reduced the rise time of the synaptic potentials. These effects were accompanied by a reduction in the Hill coefficient for Ca 2+ ‐dependence of synaptic transmission. Our findings support the hypothesis that synaptotagmin inhibits neurotransmitter release in the absence of Ca 2+ .