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Differential expression of nuclear lamin, the major component of nuclear lamina, during neurogenesis in two germinal regions of adult rat brain
Author(s) -
Takamori Yasuharu,
Tamura Yasuhisa,
Kataoka Yosky,
Cui Yilong,
Seo Saori,
Kanazawa Takenori,
Kurokawa Kiyoshi,
Yamada Hisao
Publication year - 2007
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.2007.05450.x
Subject(s) - lamin , nuclear lamina , subgranular zone , biology , neurogenesis , neun , microbiology and biotechnology , neural stem cell , stem cell , progenitor cell , subventricular zone , gliogenesis , cellular differentiation , nucleus , nuclear protein , immunohistochemistry , immunology , genetics , gene , transcription factor
Lamins are major structural proteins of the nuclear envelope. Three lamin subtypes, A/C, B1 and B2, predominate in mammalian somatic cells. While the expression levels of lamins in several tissues are known to change during cell differentiation, lamin expression is poorly understood in the nervous system. To investigate the expression of lamins during neuronal differentiation in the mammalian adult brain, we performed immunohistochemical studies on lamins A/C, B1 and B2 in two neurogenic regions of rat brain: the subgranular zone of the dentate gyrus and the subventricular zone of the lateral ventricle. In particular, three types of cells were analysed using confocal microscopy: GFAP‐positive cells as primary progenitor (stem) cells, PSA‐NCAM‐positive cells as subsequent neuronal progenitor cells, and NeuN‐positive mature neurons. GFAP‐positive cells possesed lamin A/C (++), B1 (++) and B2 (++), PSA‐NCAM‐positive cells possessed lamin A/C (–), B1 (+++) and B2 (+), and mature neurons possessed lamin A/C (++), B1 (+) and B2 (+++), in both neurogenic regions. These observations showed that the compositions of expressing lamin subtypes are distinct in particular differentiation stages during neurogenesis in the adult rat brain. Our results suggest that the alteration of nuclear lamina structure is coupled with the progression of neuronal differentiation.