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The extreme C‐terminus of GluRδ2 is essential for induction of long‐term depression in cerebellar slices
Author(s) -
Kohda Kazuhisa,
Kakegawa Wataru,
Matsuda Shinji,
Nakagami Ryoichi,
Kakiya Naomasa,
Yuzaki Michisuke
Publication year - 2007
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.2007.05412.x
Subject(s) - pdz domain , cerebellum , purkinje cell , long term depression , microbiology and biotechnology , mutant , glutamate receptor , c terminus , wild type , neuroscience , biology , chemistry , in situ hybridization , receptor , gene expression , biochemistry , ampa receptor , gene , amino acid
Long‐term depression (LTD) of parallel fibre (PF)–Purkinje cell synapses in the cerebellum is recognized as a cellular substrate of motor learning. Although the δ2 glutamate receptor (GluRδ2) has been shown to be crucial for LTD, the mechanisms by which GluRδ2 functions remain elusive. In this study, we developed a virus vector‐based gene transfer approach to rescue impaired LTD in GluRδ2 ‐null Purkinje cells in cerebellar slice preparations. We demonstrated that LTD was restored in GluRδ2 ‐null Purkinje cells transduced with wild‐type but not with mutant GluRδ2 , which lacked the PDZ‐ligand domain in the C‐terminus. Immunohistochemical analysis revealed no difference in expression levels or spine localization patterns between virally introduced wild‐type and mutant GluRδ2 proteins. Similarly, LTD was abrogated in Purkinje cells that had been acutely perfused with peptides, hampering the interaction of GluRδ2 with PDZ proteins such as PSD‐93, PTPMEG and S‐SCAM but not with delphilin. Together, these results indicate that PDZ proteins that bind to the C‐terminus of GluRδ2 are not essential for localizing GluRδ2 at synapses but are crucial for conveying signals necessary for the induction of LTD.