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Axonal transport of BDNF precursor in primary sensory neurons
Author(s) -
Wang Hui,
Wu Linda Linyan,
Song XingYun,
Luo XueGang,
Zhong JinHua,
Rush Robert A.,
Zhou XinFu
Publication year - 2006
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.2006.05138.x
Subject(s) - sciatic nerve , sensory system , neuroscience , axoplasmic transport , brain derived neurotrophic factor , spinal cord , neurotrophic factors , chemistry , anatomy , neurotrophin , biology , receptor , biochemistry
Recent studies have shown that the precursor of brain‐derived neurotrophic factor (pro‐BDNF) activates p75NTR with high affinity to induce apoptosis. Here we show that pro‐BDNF is transported anterogradely and retrogradely in sensory neurons of adult rats. After a crush injury of sciatic nerves, dorsal roots or dorsal column in adult Sprague–Dawley rats, the immunoreactivity for pro‐BDNF accumulated at both the proximal and distal segments. The accumulation reached a maximum at 24 h after injury. Western blot analysis also revealed pro‐BDNF in sciatic nerve segments proximal and distal to the ligature and in the spinal cord. Biotinylated or Alexa‐488‐labelled pro‐BDNF injected into sciatic nerve was internalized and transported both retrogradely and anterogradely within sensory neurons. These results demonstrate that pro‐BDNF is anterogradely and retrogradely transported in sensory neurons, suggesting that endogenous pro‐BDNF may be released and play important functions.