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Isolation and characterization of neural precursor cells from the Sox1 –GFP reporter mouse
Author(s) -
Barraud Perrine,
Thompson Lachlan,
Kirik Deniz,
Björklund Anders,
Parmar Malin
Publication year - 2005
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.2005.04352.x
Subject(s) - isolation (microbiology) , green fluorescent protein , characterization (materials science) , microbiology and biotechnology , chemistry , biology , biochemistry , nanotechnology , materials science , bioinformatics , gene
Abstract We have made use of a reporter mouse line in which enhanced green fluorescence protein (GFP) is inserted into the Sox1 locus. We show that the GFP reporter is coexpressed with the Sox1 protein as well as with other known markers for neural stem and progenitor cells, and can be used to identify and isolate these cells by fluorescence‐activated cell sorting (FACS) from the developing or adult brain and from neurosphere cultures. All neurosphere‐forming cells with the capacity for multipotency and self‐renewal reside in the Sox1 –GFP‐expressing population. Thus, the Sox1 –GFP reporter system is highly useful for identification, isolation and characterization of neural stem and progenitor cells, as well as for the validation of alternative means for isolating neural stem and progenitor cells. Further, transplantation experiments show that Sox1 –GFP cells isolated from the foetal brain give rise to neurons and glia in vivo , and that many of the neurons display phenotypic characteristics appropriate for the developing brain region from which the Sox1 –GFP precursors were derived. On the other hand, Sox1 –GFP cells isolated from the adult subventricular zone or expanded neurosphere cultures gave rise almost exclusively to glial cells following transplantation. Thus, not all Sox1 –GFP cells possess the same capacity for neuronal differentiation in vivo .