Premium
Loss of Gli3 enhances the viability of embryonic telencephalic cells in vitro
Author(s) -
Zaki Paulette A.,
Martynoga Ben,
DelafieldButt Jonathan T.,
Fotaki Vassiliki,
Yu Tian,
Price David J.
Publication year - 2005
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.2005.04323.x
Subject(s) - gli3 , viability assay , embryonic stem cell , biology , programmed cell death , microbiology and biotechnology , progenitor cell , in vitro , transcription factor , stem cell , apoptosis , biochemistry , gene , repressor
The transcription factor Gli3 is important for brain and limb development. Mice homozygous for a mutation in Gli3 ( Gli3 Xt/Xt ) have severe abnormalities of telencephalic development and previous studies have suggested that aberrant cell death may contribute to the Gli3 Xt/Xt phenotype. We demonstrate that telencephalic cells from embryonic Gli3 Xt/Xt embryos survive better and are more resistant to death induced by cytosine arabinoside, a nucleoside analogue that induces death in neuronal progenitors and neurons, than are control counterparts in vitro . Culture medium conditioned by Gli3 Xt/Xt cells is more effective at enhancing the viability of control telencephalic cells than medium conditioned by control cells, indicating that Gli3 Xt/Xt cells release a factor or factors which enhance telencephalic cell viability. Gli3 Xt/Xt cells are also more sensitive to released factors present in conditioned media. These data suggest that Gli3 plays both cell‐autonomous and cell‐nonautonomous roles in mediating telencephalic cell viability.