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Two‐way active avoidance training‐specific increases in phosphorylated cAMP response element‐binding protein in the dorsal hippocampus, amygdala, and hypothalamus
Author(s) -
Saha Subhash,
Datta Subimal
Publication year - 2005
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.2005.04166.x
Subject(s) - creb , amygdala , hypothalamus , hippocampus , neuroscience , endocrinology , medicine , hippocampal formation , psychology , chemistry , transcription factor , biochemistry , gene
Previous studies have demonstrated that the activation of pontine‐wave (P‐wave) generating cells in the brainstem during post‐training rapid eye movement (REM) sleep is critical for the consolidation of memory for two‐way active avoidance (TWAA) learning in the rat. Here, using immunocytochemistry, we investigated the spatio‐temporal distribution of CREB phosphorylation within different parts of the dorsal hippocampus, amygdala, and hypothalamus following a session of TWAA training in the rat. We show that the TWAA training trials increased phosphorylation of CREB (p‐CREB) in the dorsal hippocampus, amygdala, amygdalo‐hippocampal junction (AHi), and hypothalamus. However, the time intervals leading to training‐induced p‐CREB activity were different for different regions of the brain. In the dorsal hippocampus, p‐CREB activity was maximal at 90 min and this activity disappeared by 180 min. In the AHi, activity of the p‐CREB peaked by 180 min and disappeared by 360 min. In the amygdala, the p‐CREB activity peaked at 180 min and still remained higher than the control at the 360 min interval. In the hypothalamus, at 90 min p‐CREB activity was present only in the ventromedial hypothalamus; however, by 180 min this p‐CREB activity was also present in the dorsal hypothalamus, perifornical area, and lateral hypothalamus. By 360 min, p‐CREB activity disappeared from the hypothalamus. This TWAA training trials‐induced spatiotemporal characteristic of CREB phosphorylation, for the first time, suggests that REM sleep P‐wave generator activation‐dependent memory processing involves different parts of the dorsal hippocampus, amygdala, and hypothalamus.