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Coronin 3 and its role in murine brain morphogenesis
Author(s) -
Hasse Andreas,
Rosentreter André,
Spoerl Ziqiang,
Stumpf Maria,
Noegel Angelika A.,
Clemen Christoph S.
Publication year - 2005
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.2005.03917.x
Subject(s) - neurite , biology , microbiology and biotechnology , morphogenesis , dentate gyrus , hippocampal formation , activator (genetics) , neuroscience , green fluorescent protein , in vitro , receptor , genetics , gene
Abstract Coronins belong to the fundamental WD40‐repeat proteins. They are mainly found at the submembraneous area, they bind F‐actin in vitro , and most of the seven mammalian coronins have unclear roles. Coronin 3 is abundantly expressed in the adult CNS. All murine brain areas express coronin 3 during embryogenesis and the first postnatal stages. Expression in grey matter decreases postnatally, except for hippocampal pyramidal and dentate gyrus neurons, and cerebellar Purkinje cells, while levels in white matter increase in the course of myelination. Consistently, coronin 3 is abundant in differentiating neuro‐2a and PC‐12 cells and in primary oligodendrocytes. Treatment with PKC activator PMA reduced coronin 3 protein levels. To address its functions, neuro‐2a and PC‐12 cells were transfected with GFP‐tagged coronin 3 versions. Full‐length coronin 3 among other areas localized to outgrowing neurites, whereas truncated proteins efficiently suppressed neurite formation. Our results favour a role for coronin 3 in neuron morphogenesis and possibly migration.