Premium
Suppressor of cytokine signalling‐2 and epidermal growth factor regulate neurite outgrowth of cortical neurons
Author(s) -
Goldshmit Yona,
Greenhalgh Christopher J.,
Turnley Ann M.
Publication year - 2004
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.2004.03698.x
Subject(s) - neurite , socs2 , microbiology and biotechnology , epidermal growth factor , biology , cytokine , cytokine receptor , proto oncogene tyrosine protein kinase src , neuroscience , signal transduction , receptor , immunology , suppressor , in vitro , biochemistry , gene
Factors that regulate neurite outgrowth are important in determining the wiring of the central nervous system. Here we describe that the intracellular regulator of cytokine signalling, suppressor of cytokine signalling‐2 (SOCS2) and epidermal growth factor (EGF), both of which are expressed in the cortical plate during neural development, promote neurite outgrowth of cortical neurons. Cortical neurons derived from transgenic mice that over‐express SOCS2 had an increased rate of neurite outgrowth and an increased length and number of primary neurites compared with wild‐type neurons. EGF produced a similar effect in wild‐type cortical neurons and further enhanced the SOCS2‐induced neurite outgrowth. The mechanism of neurite outgrowth induction by SOCS2 and EGF at least partially overlapped as phosphorylation of the EGF receptor in SOCS2 over‐expressing or EGF‐stimulated neurons was increased on Tyrosine 845 , the Src binding site and neurite outgrowth in both protocols was blocked by inhibitors of the EGF receptor kinase and Src kinase.