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Identification of a novel brain‐specific and reelin‐regulated gene that encodes a protein colocalized with synapsin
Author(s) -
Kuvbachieva Anelia,
Bestel AudeMarie,
Tissir Fadel,
Maloum Ismahane,
Guimiot Fabien,
Ramoz Nicolas,
Bourgeois Francine,
Moalic JeanMarie,
Goffinet André M.,
Simonneau Michel
Publication year - 2004
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.2004.03473.x
Subject(s) - dab1 , reelin , moesin , reeler , biology , microbiology and biotechnology , signal transducing adaptor protein , fusion protein , radixin , olfactory bulb , neurite , ezrin , gene , neuroscience , signal transduction , genetics , cytoskeleton , recombinant dna , cell , in vitro , extracellular matrix , central nervous system
We carried out a screening of genes that are differentially expressed in normal mice and reeler mutants and are characterized by abnormal neuronal migration and neurite deployment due to defective Reelin signalling. A novel gene, provisionally named C61, was overexpressed in Reelin‐deficient embryonic mouse brain RNA. C61 encodes a 3.7 kb mRNA that is brain specific and developmentally regulated, with predominant expression in differentiating neurons. The predicted protein is 664 amino acids long, and contains LAG1 and Ezrin/Radixin/Moesin‐Myosin‐Filament motifs, suggesting that it may function as an intracellular adaptor. From E14.5 to birth, C61 was highly expressed in all neuronal differentiation fields, with the highest signal in the telencephalic cortical plate and mitral cells in the olfactory bulb. When expressed as a GFP fusion protein in transfected non‐neuronal cells and primary neurons, this protein localizes, respectively, to the nuclear membrane or axonal outgrowths, indicating a function in axonal traffic or signalling.