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Interaction of the C‐terminal tail region of the metabotropic glutamate receptor 7 with the protein kinase C substrate PICK1
Author(s) -
El Far Oussama,
Airas José,
Wischmeyer Erhardt,
Nehring Ralf B.,
Karschin Andreas,
Betz Heinrich
Publication year - 2000
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.2000.01309.x
Subject(s) - metabotropic glutamate receptor , metabotropic glutamate receptor 2 , metabotropic glutamate receptor 8 , metabotropic glutamate receptor 7 , metabotropic glutamate receptor 1 , metabotropic glutamate receptor 6 , metabotropic glutamate receptor 5 , pdz domain , biochemistry , subfamily , biology , chemistry , microbiology and biotechnology , hek 293 cells , metabotropic receptor , glutamate receptor , receptor , gene
Group III metabotropic glutamate receptors (mGluRs) are highly enriched in the presynaptic terminals of glutamatergic synapses where they mediate feedback inhibition of neurotransmitter release. Here, we used the yeast two‐hybrid system to identify a direct interaction of the C‐terminal tail region of mGluR7 with the rat homologue of the protein kinase C substrate PICK1. This interaction is specifically mediated by the very C‐terminal amino acids of the receptor and can be reconstituted in human embryonic kidney 293 cells by transfection of full‐length mGluR7 and PICK1 cDNAs. Quantitative β‐galactosidase assays revealed that among the different group III mGluRs, mGluR7 is the major PICK1 binding partner although other subfamily members can also interact with PICK1. These data indicate that PDZ domain‐containing proteins might contribute to the presynaptic localization of group III mGluRs.