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The Role of the Mouse Macrophage Scavenger Receptor in Myelin Phagocytosis
Author(s) -
Costa Cristiane Cabral,
Laan Luc J. W.,
Dijkstra Christine D.,
Brück Wolfgang
Publication year - 1997
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1997.tb01694.x
Subject(s) - scavenger receptor , phagocytosis , macrophage , myelin , biology , microbiology and biotechnology , monoclonal antibody , immunology , antibody , in vitro , biochemistry , lipoprotein , endocrinology , central nervous system , cholesterol
Myelin phagocytosis during Wallerian degeneration and immune‐mediated demyelination depends on the action of mononuclear cells of the monocyte/macrophage system. The present study investigated the role of the macrophage scavenger receptor, a trimeric membrane glycoprotein, in myelin uptake by macrophages. Two in vitro models of myelin phagocytosis were used: an organ culture model of mouse peripheral nerves exposed to cocultured macrophages and a quantitative flow cytometric assay. Different concentrations of the monoclonal rat anti‐mouse scavenger receptor antibody (2F8) were applied to these systems to selectively block the macrophage scavenger receptor. Concentration‐dependent effects on macrophage migration and myelin uptake were seen when the macrophage scavenger receptor was blocked by the antibody 2F8. Low concentrations reduced myelin phagocytosis by the invading macrophages; higher concentrations completely abolished macrophage invasion of the nerves. Using a quantitative flow cytometric assay it was also shown that the 2F8 antibody inhibits phagocytosis of myelin in a dose‐dependent manner. These data indicate that the macrophage scavenger receptor is involved in myelin phagocytosis by macrophages.