Premium
Transient Up‐regulation of Ciliary Neurotrophic Factor Receptor‐α mRNA in Axotomized Rat Septa1 Neurons
Author(s) -
Lee MunYong,
Naumann Thomas,
Kirsch Matthias,
Frotscher Michael,
Hofmann HansDieter
Publication year - 1997
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1997.tb01639.x
Subject(s) - fimbria , septal nuclei , lesion , ciliary neurotrophic factor , diagonal band of broca , cholinergic neuron , fornix , medicine , nucleus , endocrinology , neurotrophic factors , biology , neuroscience , axotomy , cholinergic , anatomy , chemistry , receptor , central nervous system , pathology , hippocampus , biochemistry , escherichia coli , gene
Using non‐radioactive in situ hybridization we investigated the effect of fimbria‐fornix transection on the expression of ciliary neurotrophic factor receptor α (CNTFRα) mRNA in axotomized septohippocampal neurons of the rat septal complex. Whereas CNTFRα expression was undetectable in the medial septal nucleus/diagonal band complex (MSDB) of control animals, specific up‐regulation was observed in MSDB neurons after fimbria‐fornix transection. CNTFRα expression was maximal 7–10 days after the lesion and had returned to control levels after 3 weeks. Following unilateral fimbria‐fornix transection, CNTFRα up‐regulation was restricted to the MSDB ipsilateral to the lesion. When cholinergic septal neurons were selectively eliminated by immunolesioning with 192 IgG‐saporin prior to fimbria‐fornix transection, the lesion‐induced expression of CNTFRα was still observed in many medial septal nucleus neurons. These results demonstrate that after fimbria‐fornix transection CNTFRα expression is transiently induced in axotomized, non‐cholinergic neurons of the medial septal nucleus, suggesting a postlesion function of locally supplied CNTF.