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Critical Role for M 3 Muscarinic Receptors in Paradoxical Sleep Generation in the Cat
Author(s) -
Sakai Kazuya,
Onoe Hirotaka
Publication year - 1997
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1997.tb01619.x
Subject(s) - methoctramine , pirenzepine , muscarinic acetylcholine receptor , carbachol , endocrinology , medicine , antagonist , locus coeruleus , muscarinic acetylcholine receptor m1 , tegmentum , muscarinic antagonist , rapid eye movement sleep , muscarinic acetylcholine receptor m3 , chemistry , psychology , receptor , neuroscience , central nervous system , electroencephalography , midbrain
In the cat, microdialysis application of 200 μM carbachol to the peri‐locus coeruleus a (peri‐LCα) of the mediodorsal pontine tegmentum produced a marked (≤5–fold) increase in paradoxical sleep. This effect was blocked by 5–50 μM 4–diphenylacetoxy‐N‐methylpiperidine methiodide (4–DAMP), an M 1 /M 3 ‐selective muscarinic receptor antagonist. In contrast, the effect was not reversed by methoctramine, an M 2 ‐selective antagonist, or pirenzepine, an M 1 ‐selective antagonist, even at concentrations as high as 500 μM. In addition, unilateral application of 5 μM 4–DAMP alone to the peri‐LCa induced both a >60% decrease in paradoxical sleep and a state of paradoxical sleep without atonia, whereas 50 μM pirenzepine and 500 μM methoctramine had no effect. Our findings are further evidence for the important role played by the peri‐LCα and demonstrate a critical role for M 3 muscarinic cholinergic receptors in the generation of paradoxical sleep.