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Molecular and Functional Diversity at Synapses of Individual Neurons In Vitro
Author(s) -
Staple Julie K.,
OsenSand Astrid,
Benfenati Fabio,
Pich Emilio Merlo,
Catsicas Stefan
Publication year - 1997
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1997.tb01420.x
Subject(s) - synapsin i , synapsin , synaptophysin , synaptic vesicle , neuroscience , synapse , neuron , biology , immunocytochemistry , neurotransmission , synaptosome , chemistry , microbiology and biotechnology , vesicle , central nervous system , biochemistry , receptor , immunohistochemistry , endocrinology , membrane , immunology
We have quantified activity‐dependent uptake of the fluorescent dye FM1–43 in combination with immunocytochemistry for synaptic vesicle‐associated proteins (SVPs) at individual synapses in primary cultures of rat cortical neurons. We show that expression of synaptic proteins is highly variable and that the levels of synaptophysin (p38), synapsin I and sv2, but not synapsin II, correlate with the extent of FM1–43 labelling at synapses. The data indicate that SVP levels affect the uptake of FMI‐43 with different efficacy (p38 > synapsin I > sv2 or synapsin 11). We also found that the relative levels of SVPs vary at individual boutons of single neurons grown in isolation, which indicates that differential regulation of specific SVPs may contribute to the selective modulation of activity at synapses of the same neuron.