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Analysis of the Ion Channel Complement of the Rat Oligodendrocyte Progenitor in a Commonly Studied In vitro Preparation
Author(s) -
Williamson A. V.,
Compston D. A. S.,
Randall A. D.
Publication year - 1997
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1997.tb01419.x
Subject(s) - patch clamp , oligodendrocyte , astrocyte , ion channel , biophysics , neuroscience , electrophysiology , chemistry , progenitor cell , voltage clamp , membrane potential , progenitor , reversal potential , microbiology and biotechnology , biology , biochemistry , central nervous system , myelin , stem cell , receptor
We have analysed the ion channel complement of the oligodendrocyte‐type 2 astrocyte (O‐2A) glial cell progenitor obtained from the commonly studied neonatal rat mixed brain preparation. Ionic currents, in O‐2A progenitors identified on both morphological and immunological grounds, were recorded using the whole‐cell variant of the patch‐clamp technique. The cells had an average resting membrane potential close to ‐50 mV and fired single action potentials in response to suprathreshold current injections. Using voltage‐clamp methods we were able to identify and characterize a voltage‐activated TTX‐sensitive Na + current, two classes of voltage‐activated outward K + currents, an inactivating inwardly rectifying K + current, a voltage‐activated Cl ‐ current and at least three classes of Ca 2+ current.