Premium
Localization, Differential Expression and Retrograde Axonal Transport Suggest Physiological Role of FGF‐2 in Spinal Autonomic Neurons of the Rat
Author(s) -
Blottner Dieter,
Stapf Christian,
Meisinger Christof,
Grothe Claudia
Publication year - 1997
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1997.tb01406.x
Subject(s) - spinal cord , neuroscience , neurotrophin , biology , axoplasmic transport , medulla , axotomy , fibroblast growth factor , autonomic nervous system , adrenal medulla , neurotrophic factors , fibroblast growth factor receptor 1 , central nervous system , microbiology and biotechnology , anatomy , endocrinology , receptor , biochemistry , heart rate , catecholamine , blood pressure
Fibroblast growth factor‐2 (FGF‐2) has marked pharmacological neurotrophic effects on lesioned spinal autonomic neurons following target removal of the adrenal medulla, yet expression and axonal transport in autonomic neurons remain to be shown. We show here FGF‐2 and FGF receptor type 1 (FGFR1) protein and mRNA expression in preganglionic intermediolateral neurons of the rat thoracic spinal cord. While immunoreactivity of both FGF‐2 and FGFR1 co‐localize to intermediolateral neurons, mRNA transcripts of FGFR1, but not of FGF‐2, are detectable in intermediolateral preparations by RNase protection analysis, suggesting protein translocation in viva Unilateral microinjection of 125 iodinated FGF‐2 into the adrenal medulla (a major target of intermediolateral neurons) results in significant accumulation of specific radioactivity in thoracic spinal cord tissue, including the intermediolateral neurons, and the ipsilateral splanchnic nerve. Emulsion autoradiography demonstrated labelling over ipsilateral intermediolateral neurons only. Neuronal co‐localization of FGF‐2/FGFR1 protein, differential mRNA expression, specific retrograde axonal transport and the known neurotrophic actions in vivo , strongly suggest unique physiological roles of FGF‐2 in the autonomic nervous system.