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Involvement of γ and β Actin Isoforms in Mouse Neuroblastoma Differentiation
Author(s) -
Ulloa Luis,
Avila Jesus
Publication year - 1996
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1996.tb01606.x
Subject(s) - neurite , actin , microbiology and biotechnology , gene isoform , neuroblastoma , biology , actin remodeling , actin remodeling of neurons , actin cytoskeleton , profilin , cytoskeleton , mdia1 , microfilament , cell , cell culture , in vitro , biochemistry , genetics , gene
Two actin isoforms, γ and β, are contained within neuroblastoma cells. However, the relative amount and distribution of both isoforms within the cells are differentially regulated during neurite extension. The proportion of γ actin isoform became about four times greater than that of β actin during neuroblastoma cell differentiation. Additionally, whereas β actin appears to be concentrated in the cell cortex, γ actin is also present throughout the cell body. Upon differentiation, neuroblastoma cells reorganize their actin cytoskeleton and γ actin is induced to polymerize whereas β actin polymers are partially disassembled. Moreover, both actin isoforms are differentially distributed within differentiated cells. Thus, γ actin polymers are located both in the soma and proximal regions of extended neurites, whereas β actin is enriched in the terminal tip of the neurites. Our results strongly suggest that both actin isoforms are involved in a different way in neuroblastoma cell differentiation.