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Induction of Stat3, a Signal Transducer and Transcription Factor, in Reactive Microglia following Transient Focal Cerebral Ischaemia
Author(s) -
Planas Anna M.,
Soriano Marc A.,
Berruezo Meritxell,
Justicia Carles,
Estrada Anna,
Pitarch Silvia,
Ferrer Isidre
Publication year - 1996
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1996.tb01556.x
Subject(s) - microglia , stat3 , cerebral ischaemia , transient (computer programming) , transcription factor , signal (programming language) , neuroscience , transducer , stat protein , ischemia , medicine , chemistry , cardiology , microbiology and biotechnology , biology , signal transduction , physics , computer science , inflammation , acoustics , gene , biochemistry , programming language , operating system
Stat3, a member of the family of cytoplasmic signal transducers and activators of transcription, was found in the rat brain in vivo under physiological conditions and was stimulated following transient focal cerebral ischaemia. A transient episode of middle cerebral artery occlusion induced a strong microglial response in the areas undergoing neural cell death from 4 days after middle cerebral artery occlusion. This was accompanied by increased expression of Stat3 in the ipsilateral cortex and striatum, as revealed by Western blotting of tissue extracts. immunohistochemistry showed strong induction of Stat3 in reactive microglial cells 4, 7 and 15 days after cerebral ischaemia. Stat3 was seen in the microglia cytoplasm, but in many microglial cells immunoreactivity was also distributed within the nucleus. These results suggest that Stat3 mediates signal transduction and activates transcription in reactive microglia in vivo following brain ischaemia.