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Potassium Channel mRNA Expression in Prevertebral and Paravertebral Sympathetic Neurons
Author(s) -
Dixon Jane E.,
McKin David
Publication year - 1996
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1996.tb01179.x
Subject(s) - superior cervical ganglion , sympathetic ganglion , protein subunit , ganglion , potassium channel , biology , electrophysiology , gene expression , gene , messenger rna , cervical ganglia , neuroscience , microbiology and biotechnology , anatomy , medicine , endocrinology , genetics
The expression of eighteen different voltage‐activated potassium channel genes in rat sympathetic ganglia was quantitatively analysed using an RNase protection assay. Eleven α‐subunit genes and two β‐subunit genes were expressed in sympathetic ganglia. The relative level of potassium channel mRNA expression was compared between the superior cervical ganglion (SCG) and two prevertebral sympathetic ganglia, the coeliac ganglion (CG) and the superior mesenteric ganglion (SMG). Four mRNAs were differentially expressed: Kv1.2, Kv1.4, Kv2.2 and Kvβ1 . Transcripts from all four genes were more abundant in the prevertebral ganglia. From comparisons with previous electrophysiological studies it was concluded that genes encoding the channels underlying the M‐current and D2‐current, which are both prominent in sympathetic neurons, have yet to be identified. It was also concluded that members of the Kv4 family are likely to underlie the low‐threshold A‐current in sympathetic neurons.