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β‐Adrenergic Receptor Activation Promotes Process Outgrowth in an Embryonic Rat Basal Forebrain Cell Line and in Primary Neurons
Author(s) -
Kwon John H.,
Eves Eva M.,
Farrell Stephen,
Segovia Jose,
Tobin Allan J.,
Wainer Bruce H.,
Downen Martha
Publication year - 1996
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1996.tb00724.x
Subject(s) - basal forebrain , embryonic stem cell , forebrain , neuroscience , microbiology and biotechnology , basal (medicine) , receptor , process (computing) , biology , endocrinology , central nervous system , genetics , gene , computer science , insulin , operating system
A clonal cell line, AS583‐8.E4.22, from the embryonic day 15 rat basal forebrain was established using retrovirus‐mediated transduction of a temperature‐sensitive mutant of the simian virus 40 (SV40) large tumour antigen. The cell line expresses cytoskeletal and neurotransmitter features indicative of neuronal commitment. In response to agents that increase intracellular CAMP, including forskolin and catecholamines, the cell line exhibits rapid process outgrowth and growth cone formation that does not require new gene expression or protein synthesis. The neurite outgrowth induced by catecholamines is mediated by β2‐adrenergic receptors and is characterized by a rapid, reversible redistribution of filamentous actin. Neurons from primary cultures of embryonic day 15 basal forebrain were also found to respond to β‐adrenergic receptor agonists by enhancing growth cone formation. These results suggest that catecholamines provide cues that induce cytoskeletal rearrangements leading to neuronal process outgrowth and growth cone formation in the developing basal forebrain and possibly other neuronal progenitor cell populations. The neuronal basal forebrain cell line provides an ideal model to study the signalling mechanisms underlying the catecholamine‐induced process outgrowth.