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Effect of Collicular Proteoglycan on the Survival of Adult Rat Retinal Ganglion Cells Following Axotomy
Author(s) -
Huxlin Krystel R.,
Dreher B.,
Schulz M.,
Sefton A. Jervie,
Bennett M. R.
Publication year - 1995
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1995.tb01024.x
Subject(s) - axotomy , retina , optic nerve , ganglion , chondroitin sulfate proteoglycan , proteoglycan , lesion , retinal ganglion cell , anatomy , retinal , neuroscience , biology , chemistry , medicine , ophthalmology , central nervous system , pathology , cartilage
Consistent with numerous previous studies, we have found that in adult rats 29% of cells retrogradely prelabelled by injections into retino‐recipient nuclei are lost 1 week after intraorbital section of the optic nerve. This figure increases to 76% 2 weeks after axotomy. Intraocular injections of 150 ng of 480 k d a chondroitin sulphate proteoglycan purified from the superior colliculi of neonatal rats were performed every third day after axotomy. This procedure resulted in the loss of only 3 and 28% of the axotomized retinal ganglion cells 7 and 14 days respectively after optic nerve section. Intraocular injections of chondroitin sulphate type C, one of the sugar types present on the collicular proteoglycan, also resulted in a significant saving of axotomized ganglion cells (with the loss of only 48% 14 days after optic nerve lesion). These findings suggest that the collicular proteoglycan, and to a lesser extent its sugar moieties, substantially slows down the degeneration of adult retinal ganglion cells following axotomy.