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Transmitter Release Associated with Long‐term Synaptic Depression in Rat Corticostriatal Slices
Author(s) -
Calabresi Paolo,
Fedele Emesto,
Pisani Antonio,
Fontana Giovanni,
Mercuri Nicola B.,
Bernardi Giorgio,
Raiteri Maurizio
Publication year - 1995
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1995.tb00710.x
Subject(s) - excitatory postsynaptic potential , tetanic stimulation , neuroscience , glutamate receptor , stimulation , long term depression , inhibitory postsynaptic potential , neurotransmission , neurotransmitter , chemistry , slice preparation , dopamine , synaptic plasticity , nmda receptor , electrophysiology , biology , biochemistry , central nervous system , ampa receptor , receptor
Using a corticostriatal slice preparation, we have recently shown that tetanic stimulation of the corticostriatal pathway produces long‐term depression (LTD) of striatal excitatory synaptic transmission. In the present study we have analysed the relationship between LTD and the striatal release of different endogenous transmitters. Samples of perfusate were collected via a small cannula placed just above the surface of the striatal slice close to the recording electrode, and were analysed by HPLC. The high‐frequency stimulation (100 Hz, three trains, 3 s duration, 20 s intervals) used to induce LTD caused a significant but transient increase in the release of both excitatory (aspartate and glutamate) and inhibitory (glycine and GABA) amino acid transmitters. Tetanic stimulation also produced a significant, but transient increase in the release of endogenous dopamine. We conclude that the tetanic stimulation of the corticostriatal pathway is able to induce a large but transient release of excitatory amino acids and of dopamine, whose participation in the induction of striatal LTD has been demonstrated previously. Moreover, the maintenance of this form of synaptic plasticity does not seem to require a sustained change in transmitter release.