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Basic Fibroblast Growth Factor Opens Calcium‐Permeable Channels in Quail Mesencephalic Neural Crest Neurons
Author(s) -
Distasi Carla,
Munaron Luca,
Laezza Fernanda,
Lovisolo Davide
Publication year - 1995
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1995.tb00348.x
Subject(s) - quail , basic fibroblast growth factor , patch clamp , neuroscience , chemistry , calcium , fibroblast growth factor , neural crest , electrophysiology , biophysics , medicine , endocrinology , biology , microbiology and biotechnology , embryo , growth factor , biochemistry , receptor , organic chemistry
In order to investigate the action of basic fibroblast growth factor (bFGF) in the nervous system, we have studied the ionic signals elicited by this peptide in cultured quail mesencephalic neural crest neurons using patch‐clamp and cytofluorimetric techniques. In this preparation stimulation with bFGF induced, with a delay of some tens of seconds, an inward cationic current. Single‐channel experiments provided evidence for the activation of a calcium‐permeable channel. In single‐cell cytofluorimetric measurements, a sustained rise in [Ca 2+ Ii was observed, which was dependent on the presence of external calcium. These events may play a role in the developmental effects of bFGF.