Premium
Expression of Soluble Guanylyl Cyclase Gene in Adult Rat Brain
Author(s) -
Burgunder J.M.,
Cheung P. T.
Publication year - 1994
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1994.tb00263.x
Subject(s) - olfactory bulb , soluble guanylyl cyclase , piriform cortex , olfactory tubercle , anterior olfactory nucleus , biology , messenger rna , striatum , gene expression , cerebellum , forebrain , granule cell , microbiology and biotechnology , in situ hybridization , neuroscience , endocrinology , central nervous system , biochemistry , nitric oxide , gene , dopamine , dentate gyrus , guanylate cyclase
The synthesis of the intracellular messenger, cyclic GMP, is catalysed by particulate or soluble guanylyl cyclase (sGCY). sGCY is activated by nitric oxide, a compound with putative neurotransmitter functions, especially in long‐term potentiation. Hybridization histochemistry with a probe complementary to the rat lung large ( α 1) subunit was used to assess the exact localization of sGCY mRNA in the rat brain. Many cells in the olfactory bulb contained sGCY mRNA. In the whole cerebral cortex, sGCY mRNA was found in all layers, with a predominance in layers II ‐ III. A similar pattern was found in the olfactory tuberculum, in continuation with the piriform cortex and the cortical amygdaloid nucleus. All parts of the striatum expressed sGCY mRNA. sGCY mRNA was also found in the habenula medialis, in the pinealis in some diencephalic nuclei, and in the granule cell layers of the cerebellum. This study provides a description of the normal anatomy of sGCY gene expression in the rat forebrain as a basis for the study of the modulation of expression after physiological and pharmacological manipulations.