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Axotomy‐induced Changes in Ca 2+ Homeostasis in Rat Sympathetic Ganglion Cells
Author(s) -
SánchezVives Maria V.,
Valdeolmillos Miguel,
Martínez Salvador,
Gallego Roberto
Publication year - 1994
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1994.tb00243.x
Subject(s) - axotomy , antidromic , superior cervical ganglion , axon , stimulation , homeostasis , chemistry , neuroscience , medicine , ganglion , sympathetic ganglion , calcium , electrophysiology , endocrinology , biology , central nervous system
Some of the marked biochemical and electrophysiological changes provoked by section of the axon in mature neurons suggest that the intracellular calcium concentration ([Ca 2+ ] i ) may be increased. We have measured the [Ca 2+ ] i using the fluorescent indicator Indo‐1 microinjected into rat superior cervical ganglion neurons. No differences in resting [Ca 2+ ] i levels were found between control neurons and cells which had been axotomized 7–10 days before. However, the rise in [Ca 2+ ] i evoked by orthodromic or antidromic stimulation and the recovery after the stimulating train were considerably slower in axotomized neurons than in control cells. We also found that the number of calbindin‐D28k‐immunopositive cells in the ganglion increases after axotomy, which could be related to the observed differences in calcium homeostasis.