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Long‐term Depression Requires Nitric Oxide and Guanosine 3′:5’Cyclic Monophosphate Production in Rat Cerebellar Purkinje Cells
Author(s) -
Daniel H.,
Hemart N.,
Jaillard D.,
Crepel F.
Publication year - 1993
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1993.tb00961.x
Subject(s) - cyclic guanosine monophosphate , nitric oxide , guanosine , cyclic gmp , chemistry , neuroscience , term (time) , depression (economics) , biology , biochemistry , physics , organic chemistry , macroeconomics , quantum mechanics , economics
In patch‐clamped Purkinje cells, bath application of the nitric oxide synthase inhibitor N G ‐methyl‐ l ‐arginine consistently prevents the induction of long‐term depression (LTD) of parallel fibre‐mediated excitatory postsynaptic potentials (EPSPs) induced by their pairing with calcium spikes. On the other hand, bath application of nitric oxide donors and of 8‐bromoguanosine 3′:5’cyclic monophosphate is able to reproduce an LTD‐like phenomenon. LTD of parallel fibre‐mediated EPSPs also occurs when nitric oxide donors or guanosine 3′:5’cyclic monophosphate are directly dialysed into Purkinje cells, and this effect partially occludes LTD induced by pairing protocols. These results show that nitric oxide does play a role in LTD induction, and demonstrate for the first time that its site of action is probably the soluble guanylate cyclase of Purkinje cells.