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Adenoviral Vector as a Gene Delivery System into Cultured Rat Neuronal and Glial Cells
Author(s) -
Caillaud Catherine,
Akli Said,
Vigne Emmanuelle,
Koulakoff Annette,
Perricaudet Michel,
Poenaru Livia,
Kahn Axel,
BerwaldNetter Yoheved
Publication year - 1993
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1993.tb00914.x
Subject(s) - biology , viral vector , recombinant dna , gene delivery , rous sarcoma virus , microbiology and biotechnology , gene , adenoviridae , cell culture , virology , transfection , genetic enhancement , virus , genetics
Previous studies have demonstrated that a defective recombinant adenovirus can infect a wide range of postmitotic and slowly proliferating cell types such as hepatocytes, myotubes, pneumocytes and intestinal cells (Stratford‐Perricaudet et al., Hum. Gene Ther. , 1 , 241–256, 1990; Quantin et al., Proc. Natl. Acad. Sci. USA , 89 , 2581–2584, 1992; Jaffe et al., Nature Genetics , 1 , 372–378, 1992). We have used a defective recombinant adenovirus, Ad.RSVβgal, containing the Escherichia coli β‐galactosidase gene targeted to the nucleus under the transcriptional control of the Rous sarcoma virus long terminal repeat promoter (Stratford‐Perricaudet et al., J. Clin. Invest. , 90 , 626–630, 1992) to infect non‐dividing neural cells in primary culture. We show that 80–100% of neuronal and astroglial cells infected with a viral titre lower than 10 9 p.f.u./ml express β‐galactosidase for at least 1 month without cell damage. These results demonstrate the potential usefulness of recombinant adenovirus infection for the analysis of brain‐specific gene regulation and for the transfer of genes into neural cells before their transplantation into the brain.