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Developmentally Regulated Expression of CGRP in the Mouse Olfactory Pathway
Author(s) -
DenisDonini S.,
Chini B.,
Vitadello M.
Publication year - 1993
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1993.tb00530.x
Subject(s) - calcitonin gene related peptide , olfactory bulb , biology , olfactory system , neuropeptide , neurofilament , in situ hybridization , anterior olfactory nucleus , neuroscience , olfactory receptor , immunohistochemistry , receptor , central nervous system , microbiology and biotechnology , messenger rna , olfactory tubercle , gene , immunology , biochemistry
The pattern of expression of the neuropeptide CGRP and its encoding mRNA has been determined by immunohistochemistry and in situ hybridization in the mouse olfactory pathway during development. Specific CGRP transcripts are first detected at E13 followed by the appearance of the peptide at E15. Both peptide and transcript are present until birth; their expression then appears to be down‐regulated since postnatally the peptide is only observed in some olfactory receptor neurons. A monoclonal antibody that specifically recognizes the neurofilament subunit NF‐M has been used in order to identify olfactory and trigeminal axons. Our results demonstrate that CGRP is expressed in olfactory neurons and their axons during development, thus supporting further its role as a differentiation factor during olfactory bulb ontogenesis.