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Pharmacological and Immunohistochemical Evidence for Serotonergic Modulation of Cholinergic Nucleus Basalis Neurons
Author(s) -
Khateb A.,
Fort P.,
Alonso A.,
Jones B. E.,
Mülethaler M.
Publication year - 1993
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1993.tb00519.x
Subject(s) - nucleus basalis , cholinergic , cholinergic neuron , basal forebrain , neuroscience , biocytin , bursting , nucleus , acetylcholine , serotonergic , biology , slice preparation , chemistry , serotonin , electrophysiology , receptor , endocrinology , biochemistry
Identified electrophysiologically by low threshold bursts and transient outward rectification, cholinergic nucleus basalis neurons were recorded and labelled intracellularly in guinea‐pig basal forebrain slices. By means of a triple labelling immunofluorescent technique, serotonin‐immunoreactive fibres were visualized in close proximity to the soma and dendrites of the biocytin‐labelled, choline acetyl transferase (ChAT)‐immunoreactive cells. By bath application, 5‐hydroxytryptamine (5‐HT) produced a direct hyperpolarization of the identified cells which was mimicked by 5‐HT 1A receptor agonists, suggesting that it may inhibit the tonic firing but also modulate the low threshold bursting of the cholinergic nucleus basalis neurons.