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Ca 2+ Channel Expression in the Oligodendrocyte Lineage
Author(s) -
Blankenfeld Gabriela v.,
Verkhratsky Alexej N.,
Kettenmann Helmut
Publication year - 1992
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1992.tb00130.x
Subject(s) - oligodendrocyte , monoclonal antibody , microbiology and biotechnology , antigen , biology , precursor cell , lineage (genetic) , chemistry , biophysics , in vitro , neuroscience , myelin , biochemistry , antibody , central nervous system , immunology , gene
The development of oligodendrocytes from their precursor cells through different developmental stages can be studied in vitro. These stages can be distinguished by specific monoclonal antibodies and by a characteristic K + channel profile. In this study we demonstrate that the occurrence of Ca 2+ currents also undergoes marked changes during the development of mouse oligodendrocytes. Immature precursor cells which can develop into astrocytes or oligodendrocytes expressed two different types of voltage‐activated Ca 2+ channels. The expression of Ca 2+ channels in precursor cells was strongly correlated with the expression of Na + channels. When cells started to express the O1 antigen and were committed to the oligodendrocyte lineage, Ca 2+ and Na + currents could no longer be detected. Large Ca 2+ currents were, however, recorded later in the development of the oligodendrocytes, correlated with the expression of the O10 antigen. The Ca 2+ channels were classified as high and low voltage‐activated Ca 2+ channels according to their range of activation, and are further described by their kinetic and pharmacological properties.