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Two GTP‐binding Proteins Control Calcium‐dependent Exocytosis in Chromaffin Cells
Author(s) -
Sontag JeanMarie,
Aunis Dominique,
Bader MarieFrance
Publication year - 1992
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1992.tb00112.x
Subject(s) - secretion , exocytosis , guanosine , microbiology and biotechnology , guanosine triphosphate , gtp' , chromaffin cell , protein kinase a , chemistry , adenosine triphosphate , biology , kinase , biochemistry , catecholamine , biophysics , adrenal medulla , endocrinology , enzyme
Abstract The effect of guanosine triphosphate analogues on catecholamine secretion from permeabilized bovine chromaffin cells was examined. Guanosine 5′‐[γ‐thio]triphosphate was demonstrated to produce a dual effect on calcium‐evoked secretion, enhancing the release through a mechanism involving protein kinase C and inhibiting secretion by a protein kinase C‐independent pathway. We propose that two functionally distinct G‐proteins control the stimulus ‐ secretion coupling in chromaffin cells.