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Behavioural Effect of Engineered Cells that Synthesize L‐DOPA or Dopamine after Grafting into the Rat Neostriatum
Author(s) -
Horellou Philippe,
Marlier Lionel,
Privat Alain,
Mallet Jacques
Publication year - 1990
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1990.tb00389.x
Subject(s) - substantia nigra , dopamine , dopaminergic , apomorphine , tyrosine hydroxylase , cell culture , oxidopamine , parkinson's disease , grafting , levodopa , neuroscience , cell , midbrain , microbiology and biotechnology , biology , chemistry , medicine , biochemistry , disease , central nervous system , genetics , organic chemistry , polymer
Cell lines in which tyrosine hydroxylase was introduced either by infection or transfection were used in grafting experiments in a rat model of Parkinson's disease obtained by unilateral lesion of the substantia nigra. A neuroblastoma NS20 Y cell line which synthesizes only L‐DOPA and a neuroendocrine AtT‐20 cell line which produces dopamine were obtained. They were grafted into denervated striata and their ability to compensate for the dopaminergic deficit was studied. Both modified cell types displayed a rapid partial reversal of apomorphine‐induced turning behaviour. No effect was observed with the control unmodified cell lines. We discuss the usefulness of engineered cell lines to address the fundamental issues in grafting experiments and more particularly in the therapy of Parkinson's disease.