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Differential Effects of Glucocorticoids on Corticotrophin‐Releasing Factor in the Rat Pituitary Neurointermediate Lobe and Median Eminence
Author(s) -
Jessop David S.,
Chowdrey Hardial S.,
Lightman Stafford L.
Publication year - 1990
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1990.tb00387.x
Subject(s) - median eminence , endocrinology , medicine , radioimmunoassay , dexamethasone , adrenalectomy , chemistry , glucocorticoid , saline , pituitary gland , hypothalamus , hormone
We have investigated the effects of glucocorticoid manipulation on corticotrophin‐releasing factor (CRF‐41) in the neurointermediate lobe (NIL) and median eminence (ME) of the rat using a radioimmunoassay specific for CRF‐41. CRF‐41 content in the NIL (88 ± 7 fmol, mean ± SEM) was not significantly altered by administration of dexamethasone in drinking water for 12 days (67 ± 9 fmol) or adrenalectomy for 12 days (96 ± 17 fmol). The saline‐stimulated increase in NIL CRF‐41 content (154 ± 24 fmol) was not affected by dexamethasone (152 ± 24 fmol) or adrenalectomy (179 ± 21 fmol). In contrast, the content of CRF‐41 in the ME declined following dexamethasone treatment (1247 ± 92 fmol to 864 ± 26 fmol), or adrenalectomy (854 ± 78 fmol). Our results provide further evidence that CRF‐41 in the NIL and ME can be differentially regulated.