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Noradrenaline‐Induced Inhibition of Voltage‐Sensitive Calcium Currents in NG108‐15 Hybrid Cells
Author(s) -
Docherty R. J.,
McFadzean I.
Publication year - 1989
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1989.tb00780.x
Subject(s) - calcium , chemistry , biophysics , voltage clamp , current (fluid) , patch clamp , membrane potential , current clamp , voltage dependent calcium channel , receptor , endocrinology , biochemistry , biology , organic chemistry , electrical engineering , engineering
Abstract The effect of noradrenaline (NA) on voltage‐sensitive calcium currents in neuroblastoma × glioma hybrid (NG108‐15) cells has been studied using a whole‐cell clamp technique. NA inhibited calcium current. The EC 50 for NA induced inhibition was 177 nM. The NA receptor involved had a similar pharmacological profile to α 2 adrenoreceptors but did not respond to clonidine. NA inhibited calcium current by an amount which depended on both membrane potential and current amplitude. When current‐dependent inactivation of calcium current was maximal, NA was without effect. The data are consistent with a mechanism where NA enhances current‐dependent inactivation.