Premium
Survival and Axonal Elongation of Adult Rat Retinal Ganglion Cells
Author(s) -
Thanos Solon,
Bähr Mathias,
Barde YvesAlain,
Vanselow Jens
Publication year - 1989
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1111/j.1460-9568.1989.tb00770.x
Subject(s) - sciatic nerve , axoplasmic transport , ganglion , anatomy , giant retinal ganglion cells , optic nerve , neurotrophic factors , immunostaining , biology , superior colliculus , neuroscience , retinal ganglion cell , retina , neurotrophin , chemistry , pathology , medicine , immunohistochemistry , biochemistry , receptor
A peripheral nerve exudate, collected in situ from the proximal end of a severed rat sciatic nerve, can induce substantial axonal elongation from ganglion cells when tested on explanted adult rat retinae. The responsive cells are identified on the basis of their Thy 1.1 immunostaining properties. Similar outgrowth can be obtained from explants when the culture medium is supplemented with brain‐derived neurotrophic factor (BDNF). In addition, both BDNF and the sciatic nerve exudate can prevent ganglion cell degeneration as shown by the retrograde transport of a fluorescent dye that had been applied to the superior colliculus prior to explantation. The results demonstrate that soluble components, released by lesioned peripheral nerves, can effect adult retinal ganglion cells in a way that is reminiscent of that which has been described in vivo using sciatic nerve grafts after sectioning of the optic nerve. The molecular nature of these components is discussed.