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Y chromosome microdeletions in Mexican males of couples with idiopathic recurrent pregnancy loss
Author(s) -
PiñaAguilar Raul E.,
MartínezGarza Sandra G.,
Kohls Graciela,
VargasMaciel Marco A.,
Vázquez de Lara Luis G.,
GonzálezOrtega Claudia,
CancinoVillarreal Patricia,
GutiérrezGutiérrez Antonio M.
Publication year - 2012
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/j.1447-0756.2011.01809.x
Subject(s) - y chromosome microdeletion , medicine , chromosome , y chromosome , pregnancy , gynecology , first trimester , genetics , obstetrics , karyotype , biology , fetus , gene
Aim: To analyze the presence of Y chromosome microdeletions in males of Mexican couples with idiopathic recurrent pregnancy losses (RPL). Methods: Seventy‐one males from couples with RPL and 66 fertile males as controls were studied. DNA was isolated from peripheral lymphocytes and used to run multiplex polymerase chain reactions. Regions AZFa (sY84, sY86), AZFb (sY127, sY134) and AZFc (sY254, sY255) of the Y chromosome were analyzed according to valid guidelines recommended by the European Academy of Andrology and the European Molecular Genetics Quality Network. Also, the sequence tagged sites (STSs): DYS262 (sY67), DYS220 (sY129), DYF85S1 (sY150), DYF86S1 (sY152) and DYF87S1 (sY153) were included in order to analyze STSs previously reported as deleted. A power analysis to support our simple size was performed. Results: Results show an absence of Y chromosome microdeletions in males of couples with RPL and controls with an acceptable statistical power. Conclusion: The study did not show an association of recurrent pregnancy loss and Y chromosome microdeletions in Mexican male partners. Based on the results, the study of Y chromosome microdeletions in couples with RPL is not considered clinically relevant.