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Effect of menstrual blood‐derived stromal stem cells on proliferative capacity of peripheral blood mononuclear cells in allogeneic mixed lymphocyte reaction
Author(s) -
Nikoo Shohreh,
Ebtekar Massoumeh,
JeddiTehrani Mahmood,
Shervin Adel,
Bozorgmehr Mahmood,
Kazemnejad Somaieh,
Zarnani Amir Hassan
Publication year - 2012
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/j.1447-0756.2011.01800.x
Subject(s) - peripheral blood mononuclear cell , medicine , mixed lymphocyte reaction , stem cell , mesenchymal stem cell , immunophenotyping , immunology , stromal cell , cd34 , lymphocyte , cd44 , andrology , t cell , flow cytometry , cell , cancer research , immune system , pathology , biology , microbiology and biotechnology , in vitro , biochemistry , genetics
Aim: Menstrual blood stromal stem cells (MBSCs) have been demonstrated to exhibit stem cell properties such as the capability for self‐renewal and multipotency, allowing for multilineage differentiation. In addition, this cell type has various immunomodulatory effects. In this study, we examined the potential effect of MBSCs on proliferation of peripheral blood mononuclear cells (PBMCs) in allogeneic mixed lymphocyte reaction (MLR). Materials and Methods: Menstrual blood was collected from healthy donors after menstrual blood flow initiated and its mononuclear cell fraction was separated. Cells were subsequently cultured and adherent cells were allowed to propagate and used as stem cells. Flowcytometric immunophenotyping was performed using a panel of monoclonal antibodies including CD44, CD45, CD34, CD9, CD29, CD10, CD38, CD105, CD73, CD133, STRO‐1 and Oct‐4A. For functional analysis, PBMCs were co‐cultured with MBSCs, collected after 4 days and added to allogeneic PBMCs. 2,3‐Bis‐(2‐methoxy‐4‐nitro‐5‐sulfophenyl)‐2H‐tetrazolium‐5‐carboxanilide (XTT) assay was carried out to evaluate cell proliferation. Results: MBSCs showed surface and intracellular markers of mesenchymal stem cells with the exception of the high expression of Oct‐4A. MBSCs affected the proliferative response of PBMC in a dose‐dependent manner. At ratio of 1:1 to 1:2, MBSCs inhibited, while at lower ratios (1:32 to 1:64) stimulated the proliferative capacity of allogeneic PBMCs. Conclusion: According to the present study, MBSCs exert their immunoregulatory effects on allogeneic PBMCs in a dose‐dependent manner. This finding can be considered as a valuable point in future cell therapy strategies, when this cell population is used.