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Effects of preeclampsia on the yield of hematopoietic stem cells obtained from umbilical cord blood at delivery
Author(s) -
Abdul Wahid Fadilah S.,
Nasaruddin Mona Zaria,
Mohd Idris Mohd Razif,
Tusimin Maiza,
Tumian Nor Rafeah,
Abdullah Mahdy Zaleha
Publication year - 2012
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/j.1447-0756.2011.01740.x
Subject(s) - cord blood , umbilical cord , medicine , cd34 , haematopoiesis , stem cell , hematopoietic stem cell , gestational age , preeclampsia , andrology , obstetrics , blood volume , cord , birth weight , blood cell , placenta cord banking , pregnancy , immunology , placenta , fetus , surgery , biology , genetics
Aim: To compare the numbers of cord blood CD34 + hematopoietic stem cells (HSC) between preeclampsia (PE) and control (non‐PE) subjects and to determine the factors that may influence this observation. Methods: Umbilical cord blood was collected from 28 PE and 19 non‐PE subjects. Nucleated and CD34 + cell counts were derived using the Trucount tube‐based stem cell enumeration kit on BD FACSCalibur. Results: The cord blood volume, nucleated and CD34 + cell counts were significantly reduced in PE subjects compared to non‐PE subjects. Among the PE subjects, systolic and diastolic blood pressure demonstrated a negative correlation with total nucleated and CD34 + cell counts. Gestational age at delivery influenced cord blood volume and nucleated cell counts, but not CD34 + cell counts. Birth weight and placental weight correlated strongly with cord blood volume, and nucleated and CD34 + cell counts. There were no correlations observed between cord blood parameters and maternal age, maternal white cell count, gravidity, route of delivery or neonatal gender among PE subjects. Conclusion: Preeclampsia has a negative impact on the yield of HSC obtained from cord blood at delivery. Maternal blood pressure, neonatal birth weight and placental weight are important factors influencing the numbers of cord blood HSC. These findings should be taken into consideration when selecting cord blood units from mothers with PE for banking. Selecting the heaviest term neonate might improve the yield of cord blood HSC obtained from PE mothers.