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Identification of protein marker in vaginal wall tissues of women with stress urinary incontinence by protein chip array
Author(s) -
Wen Yan,
Whitin John,
Yu Tom,
Cohen Harvey,
Polan Mary Lake,
Chen Bertha
Publication year - 2012
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/j.1447-0756.2011.01690.x
Subject(s) - immunohistochemistry , western blot , blot , biomarker , medicine , mass spectrometry , proteome , proteomics , protein expression , microbiology and biotechnology , biomarker discovery , urinary system , pathology , biology , chromatography , chemistry , biochemistry , gene
Aim: We sought to investigate protein biomarkers for stress urinary incontinence (SUI) in vaginal tissues using surface‐enhanced laser desorption/ionization time‐of‐flight mass spectrometry (SELDI‐TOF MS) and examine if this is a reliable methodology to examine proteins in small tissue specimens. Material and Methods: We compared protein expression profile of vaginal tissue from women with SUI and continent controls. A 22.6 kDa peak was identified by subsequent weak cation‐exchange, reverse‐phase fractionation, gel electrophoresis, and trypsin digestion, then analyzed by matrix assisted laser desorption/ionization mass spectrometry (MALDI MS) and MALDI MS‐MS. Biomarker identity and expression level were confirmed by Western‐blotting and immunohistochemistry. Results: Expression of the 22.6 kDa protein, identified as SM‐22α, was significantly higher in women with SUI versus controls. A 3 × 3‐mm tissue sample was sufficient for identification. Western‐blot/immunohistochemistry confirmed the SELDI‐TOS MS findings. Conclusion: SM‐22α, a marker for myofibroblasts, was identified as a biomarker of SUI. Differential protein profiling by SELDI‐TOF MS is a powerful and reliable tool for urogynecological research as it allows us to study an array of proteins simultaneously using small tissue samples.