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Effect of human chorionic gonadotrophin on in vitro contractions of stimulated detrusor muscle strips of female rats
Author(s) -
Rizk Diaa E. E.,
Osman Nawal A.,
Shafiullah Mohammed M.,
Nagelkerke Nicolaas J. D.,
Fahim Mohammed A.
Publication year - 2009
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/j.1447-0756.2009.01052.x
Subject(s) - carbachol , medicine , endocrinology , detrusor muscle , purinergic receptor , isometric exercise , stimulation , adenosine triphosphate , adenosine , chemistry , urinary bladder
Aims: We studied the effect of human chorionic gonadotrophin (hCG) on the in vitro detrusor muscle contractions in female rats. Methods: Two adjacent detrusor muscle strips from the bladder dome of 18 female Wistar rats (230–250 gm) were mounted in an organ bath for the recording of isometric tension. Carbachol (10 −9 –10 −3 M), α,β methylene adenosine 5′‐triphosphate (ATP) (10 −9 –10 −3 M) and potassium chloride (KCl) (10 −4 –10 −3 M) were applied ( n = 6 × 3 groups). Concentration‐response curves, before and after the addition of hCG (100 iu/mL) or oxybutynin (10 −5 M) to either muscle strip, were compared. Results: All curves were displaced to the right by hCG in a concentration‐dependent manner with significant inhibition of contractions induced by carbachol ( P < 0.001) and KCl ( P = 0.016) but not those induced by α,β‐methylene ATP ( P = 0.4). Estimated order of potency of inhibition was carbachol>KCl>α,β‐methylene ATP. The overall inhibitory effect of hCG was significantly less than oxybutynin ( P < 0.001). Conclusions: hCG significantly inhibited in vitro detrusor contractions induced by depolarization (KCl) and cholinergic (carbachol) but not purinergic (α,β‐methylene ATP) stimulation in a dose‐dependent manner in female rats.