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Protective effect of aprotinin on ischemia–reperfusion injury in rat ovary
Author(s) -
Sahin Figen Kir,
Cosar Emine,
Koken Gulengul,
Toy Hatice,
Basarali Kemal,
Buyukbas Sadik
Publication year - 2008
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/j.1447-0756.2008.00846.x
Subject(s) - aprotinin , medicine , malondialdehyde , superoxide dismutase , reperfusion injury , saline , ischemia , nitric oxide , anesthesia , endocrinology , oxidative stress
Aim: We investigated the effects of aprotinin on reperfusion injury in a controlled experimental rat torsion–detorsion model. Methods: Thirty‐two female Sprague–Dawley rats were divided into four groups. Sham operation was performed in group I; in group II only ovarian torsion was performed. In group III, torsion–detorsion was performed, plus 3 mL/kg saline was injected i.v. 30 min before detorsion. In group IV, torsion–detorsion was performed, plus aprotinin (30 000 KIU/kg) was injected 30 min before detorsion. Rats in the torsion group were killed after 360° clockwise adnexial torsion for 3 h, and the ovaries were harvested. After 3 h of adnexial detorsion, the rats in the saline and aprotinin groups were killed and the adnexa were surgically removed. Results: Ovarian tissue damage scores were significantly different among groups. Ovarian tissue and serum malondialdehyde levels in group III were significantly higher than those of groups I and IV ( P < 0.05). The serum levels of superoxide dismutase in group III were significantly lower than those of groups I and IV ( P = 0.01). Tissue and serum xanthine oxidase, nitric oxide, and tissue superoxide dismutase levels were comparable among groups ( P > 0.05). Conclusions: Aprotinin attenuates ischemia–reperfusion injury in a rat adnexial torsion–detorsion model.